Porcine Testin,TES ELISA KIT

Porcine Testin, TES ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests

Alternative name

Porcine TES ELISA KIT ,Porcine Testin ELISA KIT ,Porcine TESS ELISA KIT ,Porcine TESS-2 ELISA KIT ,Porcine testis derived transcript (3 LIM domains) ELISA KIT ,Porcine testin LIM domain protein ELISA KIT

Intended use

Porcine TES ELISA kit allows for the in vitro quantitative determination of Porcine Testin concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Porcine TES ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TES, During the reaction, TES in the sample or standard competes with a fixed amount of biotin-labeled TES for sites on a pre-coated Monoclonal antibody specific to TES. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TES in the samples is then determined by comparing the O.D. of the samples to the standard curve.