Porcine Muscarinic acetylcholi

Porcine Muscarinic acetylcholine receptor M2, CHRM2 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

CHRM2,HM2; 7TM receptor; acetylcholine receptor, muscarinic 2; muscarinic M2 receptor; muscarinic acetylcholine receptor M2; cholinergic receptor, muscarinic 2

Search name

Porcine CHRM2 ELISA KIT ,Porcine HM2 ELISA KIT ,Porcine 7TM receptor ELISA KIT ,Porcine acetylcholine receptor ELISA KIT ELISA KIT ,Porcine muscarinic 2 ELISA KIT ,Porcine muscarinic M2 receptor ELISA KIT ,Porcine muscarinic acetylcholine receptor M2 ELISA KIT ,Porcine cholinergic receptor ELISA KIT ELISA KIT ,Porcine muscarinic 2 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Porcine Muscarinic acetylcholine receptor M2, CHRM2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to CHRM2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CHRM2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CHRM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CHRM2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.