Porcine CD247 ELISA KIT

Porcine T- cell surface glycoprotein CD3 zeta, CD247 ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

CD247,CD247 molecule; CD247 antigen, zeta subunit; CD3-ZETA; CD3H; CD3Q; CD3Z; T3Z; TCRZ; CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 zeta chain; T-cell surface glycoprotein CD3 zeta chain; TCR zeta chain

Search name

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Intended use

This immunoassay kit allows for the in vitro quantitative determination of Porcine T- cell surface glycoprotein CD3 zeta, CD247 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with an antibody specific to Porcine T- cell surface glycoprotein CD3 zeta, CD247, During the reaction, Porcine T- cell surface glycoprotein CD3 zeta, CD247 in the sample or standard competes with a fixed amount of biotin-labeled Porcine T- cell surface glycoprotein CD3 zeta, CD247 for sites on a pre-coated Monoclonal antibody specific to Porcine T- cell surface glycoprotein CD3 zeta, CD247. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Porcine T- cell surface glycoprotein CD3 zeta, CD247 in the samples is then determined by comparing the O.D. of the samples to the standard curve.