Porcine esterase D,ESD ELISA K

Porcine esterase D,ESD ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY!

Alternative name

Porcine ESD ELISA KIT ,Porcine FGH ELISA KIT ,Porcine esterase 10 ELISA KIT ,Porcine esterase D/formylglutathione hydrolase ELISA KIT ,Porcine methylumbelliferyl-acetate deacetylase ELISA KIT ,Porcine testicular tissue protein Li 66 ELISA KIT ,Porcine esterase D ELISA KIT ,Porcine S-formylglutathionehydrolase ELISA KIT

Intended use

Porcine ESD ELISA KIT allows for the in vitro quantitative determination of porcine ESD concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Porcine ESD ELISA KIT has been pre-coated with an antibody specific to ESD. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forESD and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ESD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ESD in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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