Porcine Appetite-regulating ho

Porcine Appetite-regulating hormone, GHRL ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Appetite-regulating hormone,Protein M46,Motilin-related peptide,Growth hormone secretagogue,Growth hormone-releasing peptide, GHRL,ghrelin,MTLRP; appetite-regulating hormone; ghrelin, growth hormone secretagogue receptor ligand; ghrelin/obestatin preprohormone; growth hormone secretagogue; growth hormone-releasing peptide; motilin-related peptide; prepro-appetite regulatory hormone; ghrelin/obestatin prepropeptide

Search name

Porcine Appetite-regulating hormone ELISA KIT ,Porcine Protein M46 ELISA KIT ,Porcine Motilin-related peptide ELISA KIT ,Porcine Growth hormone secretagogue ELISA KIT ,Porcine Growth hormone-releasing peptide ELISA KIT ,Porcine GHRL ELISA KIT ,Porcine ghrelin ELISA KIT ,Porcine MTLRP ELISA KIT ,Porcine appetite-regulating hormone ELISA KIT ,Porcine ghrelin ELISA KIT ,Porcine growth hormone secretagogue receptor ligand ELISA KIT ,Porcine ghrelin/obestatin preprohormone ELISA KIT ,Porcine growth hormone secretagogue ELISA KIT ,Porcine growth hormone-releasing peptide ELISA KIT ,Porcine motilin-related peptide ELISA KIT ,Porcine prepro-appetite regulatory hormone ELISA KIT ,Porcine ghrelin ELISA KIT,Porcine obestatin prepropeptide ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Porcine Appetite-regulating hormone concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Appetite-regulating hormone, During the reaction, Appetite-regulating hormone in the sample or standard competes with a fixed amount of biotin-labeled Appetite-regulating hormone for sites on a pre-coated Monoclonal antibody specific to Appetite-regulating hormone. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Appetite-regulating hormone in the samples is then determined by comparing the O.D. of the samples to the standard curve.