Porcine Interferon epsilon,IFN

Porcine Interferon epsilon, IFNE ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

IFNE,IFN-E; IFNE1; IFNT1; INFE1; PRO655; IFN-epsilon; interferon epsilon; interferon epsilon 1; interferon epsilon-1; interferon tau-1; interferon-epsilon; interferon, epsilon

Search name

Porcine IFNE ELISA KIT ,Porcine IFN-E ELISA KIT ,Porcine IFNE1 ELISA KIT ,Porcine IFNT1 ELISA KIT ,Porcine INFE1 ELISA KIT ,Porcine PRO655 ELISA KIT ,Porcine IFN-epsilon ELISA KIT ,Porcine interferon epsilon ELISA KIT ,Porcine interferon epsilon 1 ELISA KIT ,Porcine interferon epsilon-1 ELISA KIT ,Porcine interferon tau-1 ELISA KIT ,Porcine interferon-epsilon ELISA KIT ,Porcine interferon epsilon ELISA KIT

Intended use

Porcine IFNE ELISA KIT allows for the in vitro quantitative determination of Porcine Interferon epsilon, IFNE concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Porcine IFNE ELISA KIT has been pre-coated with an antibody specific to Interferon epsilon. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Interferon epsilon and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Interferon epsilon, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Interferon epsilon in the samples is then determined by comparing the O.D. of the samples to the standard curve.