Porcine Creatine kinase B-type

Porcine Creatine kinase B- type, CKB ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Creatine kinase B-type,B-CK,Creatine kinase B chain, creatine kinase, brain; creatine kinase-B; creatine kinase B-type; creatine kinase B
chain; B-CK; CKBB,CKB

Search name
Porcine Creatine kinase B-type ELISA KIT ,Porcine B-CK ELISA KIT ,Porcine Creatine kinase B chain ELISA KIT ,Porcine  creatine kinase ELISA KIT ,Porcine  brain ELISA KIT ,Porcine creatine kinase-B ELISA KIT ,Porcine creatine kinase B-type ELISA KIT ,Porcine creatine kinase B chain ELISA KIT ,Porcine B-CK ELISA KIT ,Porcine CKBB ELISA KIT ,Porcine CKB ELISA KIT

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Porcine creatine kinase b-type,Creatine kinase B-type
concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Creatine kinase B-type. Standards or samples are
then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Creatine kinase
B-type and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate
solution is added to each well. Only those wells that contain Creatine kinase B-type, biotin-conjugated antibody and enzyme-conjugated
Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color
change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Creatine kinase B-type in the
samples is then determined by comparing the O.D. of the samples to the standard curve.