Porcine Complement C5a anaphyl

Porcine Complement C5a anaphylatoxin, C5 ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests

Alternative name

Porcine C5 ELISA KIT ,Porcine C5D ELISA KIT ,Porcine C5a ELISA KIT ,Porcine C5b ELISA KIT ,Porcine CPAMD4 ELISA KIT ,Porcine ECLZB ELISA KIT ,Porcine C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 ELISA KIT ,Porcine C5a anaphylatoxin ELISA KIT ,Porcine anaphylatoxin C5a analog ELISA KIT ,Porcine prepro-C5 ELISA KIT ,Porcine complement component 5 ELISA KIT

Intended use

Porcine C5 ELISA kit allows for the in vitro quantitative determination of Porcine C5 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Porcine C5 ELISA kit has been pre-coated with an antibody specific to C5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for C5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain C5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of C5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.