Porcine Neuromedin-U-25,NMU EL

Porcine Neuromedin- U- 25, NMU ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Neuromedin-U, prepro-NMU; neuromedin-U,Nmu

Search name

Porcine NMU ELISA Kit, Porcine Neuromedin-U ELISA Kit, Porcine Neuromedin U ELISA Kit, Porcine prepro-NMU ELISA Kit

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Porcine Neuromedin-U concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuromedin-U. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Neuromedin-U and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Neuromedin-U, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Neuromedin-U in the samples is then determined by comparing the O.D. of the samples to the standard curve.