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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSMBIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测猪血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
猪
- 标记物:
Porcine Alpha-1-antitrypsin,SERPINA1
- 样本:
猪血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Porcine Alpha-1-antiproteinase,Serpina1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY!
Alternative name
Porcine SERPINA1 ELISA KIT,Porcine Alpha-1-antitrypsin ELISA KIT ,Porcine Serpin A1 ELISA KIT ,Porcine Alpha-1 protease inhibitor ELISA KIT ,Porcine Alpha-1-antiproteinase ELISA KIT , Porcine A1A ELISA KIT ,Porcine A1AT ELISA KIT ,Porcine AAT ELISA KIT ,Porcine PI1 ELISA KIT ,Porcine PRO2275 ELISA KIT ,Porcine alpha1AT ELISA KIT ,Porcine alpha-1 protease inhibitor ELISA KIT ,Porcine alpha-1-antiproteinase ELISA KIT ,Porcine alpha-1-antitrypsin ELISA KIT ,Porcine alpha-1-antitrypsin null ELISA KIT ,Porcine protease inhibitor 1 (anti-elastase) alpha-1-antitrypsin ELISA KIT ,Porcine serine proteinase inhibitor clade A member 1 ELISA KIT ,Porcine serpin A1 ELISA KIT ,Porcine serpin peptidase inhibitor ELISA KIT ,Porcine clade A (alpha-1 antiproteinase ELISA KIT ,Porcine antitrypsin) member 1 ELISA KIT ,Porcine cysteine proteinase inhibitor clade A member 1 ELISA KIT
Intended use
Porcine SERPINA1 ELISA KIT allows for the in vitro quantitative determination of porcine Alpha-1-antiproteinase concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-antiproteinase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forAlpha-1-antiproteinase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Alpha-1-antiproteinase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Alpha-1-antiproteinase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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