Porcine Cadherin-5,CDH5 ELISA

Porcine Cadherin-5, CDH5 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

CDH5,Cadherin-5,7B4 antigen,Vascular endothelial cadherin,VE-cadherin,VE cadherin,cd144 antigen; CD144; 7B4; 7B4 antigen; VE-cadherin; cadherin 5 type 2, VE-cadherin vascular epithelium; cadherin-5; endothelial-specific cadherin; vascular endothelial cadherin; cadherin 5 type 2 vascular endothelium

Search name

Porcine CDH5 ELISA KIT ,Porcine Cadherin-5 ELISA KIT ,Porcine 7B4 antigen ELISA KIT ,Porcine Vascular endothelial cadherin ELISA KIT ,Porcine VE-cadherin ELISA KIT ,Porcine VE cadherin ELISA KIT ,Porcine cd144 antigen ELISA KIT ,Porcine CD144 ELISA KIT ,Porcine 7B4 ELISA KIT ,Porcine 7B4 antigen ELISA KIT ,Porcine VE-cadherin ELISA KIT ,Porcine cadherin 5 type 2 ELISA KIT ,Porcine  VE-cadherin vascular epithelium ELISA KIT ,Porcine cadherin-5 ELISA KIT ,Porcine endothelial-specific cadherin ELISA KIT ,Porcine vascular endothelial cadherin ELISA KIT ,Porcine cadherin 5 type 2 vascular endothelium ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Porcine Cadherin-5 concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cadherin-5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cadherin-5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cadherin-5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cadherin-5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.