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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSMBIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测猪血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
猪
- 标记物:
Porcine von Willebrand factor,VWF
- 样本:
猪血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Porcine von Willebrand factor,vWF ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
von Willebrand factor(vWF),; von Willebrand factor,F8VWF; VWD; coagulation factor VIII VWF
Search name
Porcine von Willebrand factor ELISA Kit, Porcine vWF ELISA Kit, von Willebrand factor ELISA Kit, vWF ELISA Kit, Porcine
F8VWF ELISA Kit, Porcine VWD ELISA Kit, Porcine coagulation factor VIII VWF ELISA Kit, F8VWF ELISA Kit, VWD ELISA Kit,
coagulation factor VIII VWF ELISA Kit,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Porcine von willebrand factor,vWF concentrations in serum,
Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to vWF. Standards or samples are then added to the
appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for vWF and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain vWF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of vWF in the samples is then determined by comparing the
O.D. of the samples to the standard curve.
Porcine-von-Willebrand-factor-vWF-ELISA-Kit.pdf">
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文献和实验Multimeric Analysis of von Willebrand Factor
Von Willebrand Factor (vWF) in normal plasma is composed of a series of high molecular multimers, ranging in size from 8�105 to over 15�106 Daltons (1 ). The multimeric structure of vWF was first investigated by two-dimensional crossed
Screening for Candidate Mutations Causing von Willebrands Disease (vWD)
von Willebrand factor (vWF) is a large, complex glycoprotein that exists in plasma and platelets, and is synthesized by megakaryocytes and endothelial cells. vWF plays an essential role in hemostasis in at least two ways. It is involved
-Duistermaat j.j,et.al. Familial clustering of factor Ⅷ and von Willebrand Factor levels , Thromb Haemost 1998,79:323-7 2.O , Donnell J,Mumford A.D,Manning R,A,Elevation of F Ⅷ :C in venous thrombembolism is persistent and independent of the Axute
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