- ￥3480
- LSMBIO
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E0592p
- 2025年07月03日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSMBIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测猪血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
猪
- 标记物：
Porcine Somatostatin
- 样本：
猪血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Porcine SOMATOSTATIN ELISA KIT
Packing 96Tests
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Gene Name SST
Protein Name SOMATOSTATIN
Alternative Name
Porcine SOMATOSTATIN ELISA KIT , Porcine SST ELISA KIT ,Porcine somatostatin-28 ELISA KIT ,Porcine somatostatin-14 ELISA KIT ,Porcine prepro-somatostatin ELISA KIT ,Porcine SMST ELISA KIT ,Porcine growth hormone release-inhibiting factor ELISA KIT
Intended use
Porcine SOMATOSTATIN ELISA KIT allows for the in vitro quantitative determination of SOMATOSTATIN concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
| Reagent | Quantity |
| Assay plate | 1 |
| Standard | 2 |
| Sample Diluent | 1 × 20mL |
| Assay Diluent A | 1 × 10mL |
| Assay Diluent B | 1 × 10mL |
| Detection Reagent A | 1 × 120μL |
| Detection Reagent B | 1 × 120μL |
| Wash Buffer(25 x concentrate) | 1 × 30mL |
| Substrate | 1 × 10mL |
| Stop Solution | 1 × 10mL |
| Plate sealer | 5 |
Test principle
The microtiter plate provided in Porcine SOMATOSTATIN ELISA KIT has been pre-coated with an SOMATOSTATIN antibody specific to SOMATOSTATIN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for SOMATOSTATIN and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SOMATOSTATIN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SOMATOSTATIN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample collection and storage
Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20
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