- ￥3480
- LSMBIO
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E0525p
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSMBIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测猪血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
猪
- 标记物：
Porcine Tissue-type Plasiminogen Actilyse
- 样本：
猪血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Porcine Tissue-type Plasiminogen Actilyse ELISA KIT
Packing 96Tests
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Gene Name T-PA
Protein Name Tissue-type Plasiminogen Actilyse
Alternative Name
Porcine Tissue- type Plasiminogen Actilyse ELISA KIT ,Porcine PLAT ELISA KIT ,Porcine T-PA ELISA KIT ,Porcine TPA ELISA KIT ,Porcine tissue-type plasminogen activator ELISA KIT ,Porcine alteplase ELISA KIT ,Porcine plasminogen/activator kringle ELISA KIT ,Porcine reteplase ELISA KIT ,Porcine t-plasminogen activator ELISA KIT ,Porcine plasminogen activator tissue type ELISA KIT
Intended use
Porcine Tissue-type Plasiminogen Actilyse ELISA KIT allows for the in vitro quantitative determination of Tissue-type Plasiminogen Actilyse concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
| Reagent | Quantity |
| Assay plate | 1 |
| Standard | 2 |
| Sample Diluent | 1 × 20mL |
| Assay Diluent A | 1 × 10mL |
| Assay Diluent B | 1 × 10mL |
| Detection Reagent A | 1 × 120μL |
| Detection Reagent B | 1 × 120μL |
| Wash Buffer(25 x concentrate) | 1 × 30mL |
| Substrate | 1 × 10mL |
| Stop Solution | 1 × 10mL |
| Plate sealer | 5 |
Test principle
The microtiter plate provided in Porcine Tissue-type Plasiminogen Actilyse ELISA KIT has been pre-coated with an Tissue-type Plasiminogen Actilyse antibody specific to Tissue-type Plasiminogen Actilyse. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Tissue-type Plasiminogen Actilyse and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Tissue-type Plasiminogen Actilyse, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Tissue-type Plasiminogen Actilyse in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample collection and storage
Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
Tissue sectioning: Cryostat sectioning method
briefly in PBS and soak in either 15% cold water fish gelatin/15% sucrose in PBS overnight or in 7.5% porcine gelatin (300 Bloom) 15% sucrose in PBS for 6 h at 37oC. * Embed in 15% sucrose/7.5% gelatin, chill to 4oC (you can hold the samples
Tissue sectioning: Cryostat sectioning method
% sucrose in PBS overnight or in 7.5% porcine gelatin (300 Bloom) 15% sucrose in PBS for 6 h at 37oC. * Embed in 15% sucrose/7.5% gelatin, chill to 4oC (you can hold the samples here for a few days). * Remove sucrose/gelatin solution and add Tissue
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