- ￥3480
- LSMBIO
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E0182p
- 2025年07月20日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSMBIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测猪血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
猪
- 标记物：
Porcine Hyaluronic acid,HA
- 样本：
猪血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Porcine Hyaluronic acid, HA ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Hyaluronic acid,Hyaluronan,Hyaluronate,;
Search name
Porcine Hyaluronic acid ELISA Kit, Porcine HA ELISA Kit,Hyaluronic acid ELISA Kit,HA ELISA Kit
Intended use
Porcine Hyaluronic acid ELISA Kit allows for the in vitro quantitative determination of general hyaluronic acid,Hyaluronic acid concentrations in serum,
plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
Porcine Hyaluronic acid ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Hyaluronic acid, During the reaction, Hyaluronic acid in the sample or standard competes with a
fixed amount of biotin-labeled Hyaluronic acid for sites on a pre-coated Monoclonal antibody specific to Hyaluronic acid. Excess conjugate
and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each
microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the
addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The
concentration of Hyaluronic acid in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Article: Escitalopram Ameliorates Cardiomyopathy in Type 2 Diabetic Rats via Modulation of Receptor for Advanced Glycation End Products and Its Downstream Signaling Cascades Author：A. Ahmed*, A. Shiha and Amina S. Attia Department of Pharmacology and Toxicology, Faculty of Pharmacy, Cairo University, Cairo, Egypt Front. Pharmacol., 15 December 2020 | doi.org/10.3389/fphar.2020.579206
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
tissue cells and fibers are embedded is a complex mixture of various glycoproteins, the most common being hyaluronic acid, chondroitin sulfate A, B, and C and keratin sulfate. Each of these glycoproteins is an unbranching polymer of two different
Tissue sectioning: Cryostat sectioning method
% ethanol : 20% DMSO (can be stored at r.t. forever). eDents bleach: 1 part 30% hydrogen peroxide & 2 parts eDents.MAB: 0.1 M maleic acid 0.15 M NaCl Adjust to pH 7.5 with NaOH Blocking Buffer: Dilute blocking solution into MAB
技术资料