- ￥3480
- LSMBIO
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E0133p
- 2025年07月20日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSMBIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测猪血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
猪
- 标记物：
Porcine tumor necrosis factor Alpha,TNFA
- 样本：
猪血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Porcine Tumor necrosis factor, TNFA ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Tumor necrosis factor,Tumor necrosis factor ligand superfamily member 2,TNF-a,Cachectin,TNF-alpha, TNF-alpha,TNFA; DIF; TNFSF2; APC1 protein; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; cachectin; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha; tumor necrosis factor
Search name
Porcine Tumor necrosis factor ELISA KIT ,Porcine Tumor necrosis factor ligand superfamily member 2 ELISA KIT ,Porcine TNF-a ELISA KIT ,Porcine Cachectin ELISA KIT ,Porcine TNF-alpha ELISA KIT ,Porcine TNF-alpha ELISA KIT ,Porcine TNFA ELISA KIT ,Porcine DIF ELISA KIT ,Porcine TNFSF2 ELISA KIT ,Porcine APC1 protein ELISA KIT ,Porcine TNF ELISA KIT ,Porcine macrophage-derived ELISA KIT ,Porcine TNF ELISA KIT ,Porcine monocyte-derived ELISA KIT ,Porcine TNF-a ELISA KIT ,Porcine cachectin ELISA KIT ,Porcine tumor necrosis factor ligand superfamily member 2 ELISA KIT ,Porcine tumor necrosis factor-alpha ELISA KIT ,Porcine tumor necrosis factor ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Porcine tumor necrosis factor,Tumor necrosis factor concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor necrosis factor. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Tumor necrosis factor and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Tumor necrosis factor, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Tumor necrosis factor in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验1975年Carswell等发现接种BCG的小鼠注射LPS后，血清中含有一种能杀伤某些肿瘤细胞或使体内肿瘤组织发生出血坏死的因子，称为肿瘤坏死因子(tumor necrosis factor，TNF)。1985年Shalaby把巨噬细胞产生的TNF命名为TNF-α，把T淋巴细胞产生的淋巴毒素(1ymphotoxin，LT)命名为TNF-β。TNF—α又称恶质素。 1．TNF的产生 (1)TNF-α是一种单核因子，主要由单核细胞和巨噬细胞产生，LPS是较强的刺激剂。IFN—r、M—CSF
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