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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测兔血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
兔
- 标记物:
Rabbit Solute carrier family 13 member 2,SLC13A2
- 样本:
兔血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rabbit SLC13A2 ELISA KIT
Packing 96Tests
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Gene Name SLC13A2
Protein Name Solute carrier family 13 member 2
Alternative Name
Rabbit SLC13A2 ELISA KIT ,Rabbit NADC1 ELISA KIT ,Rabbit NaCT ELISA KIT ,Rabbit NaDC-1 ELISA KIT ,Rabbit SDCT1 ELISA KIT ,Rabbit solute carrier family 13 member 2 ELISA KIT ,Rabbit Na(+)-coupled citrate transporter ELISA KIT ,Rabbit Na(+)/dicarboxylate cotransporter 1 ELISA KIT ,Rabbit renal sodium/dicarboxylate cotransporter ELISA KIT ,Rabbit solute carrier family 13 (sodium-dependent dicarboxylate transporter) ELISA KIT ELISA KIT ,Rabbit member 2 ELISA KIT
Intended use
Rabbit SLC13A2 ELISA KIT allows for the in vitro quantitative determination of SLC13A2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
| Reagent | Quantity |
| Assay plate | 1 |
| Standard | 2 |
| Sample Diluent | 1 × 20mL |
| Assay Diluent A | 1 × 10mL |
| Assay Diluent B | 1 × 10mL |
| Detection Reagent A | 1 × 120μL |
| Detection Reagent B | 1 × 120μL |
| Wash Buffer(25 x concentrate) | 1 × 30mL |
| Substrate | 1 × 10mL |
| Stop Solution | 1 × 10mL |
| Plate sealer | 5 |
Test principle
The microtiter plate provided in Rabbit SLC13A2 ELISA KIT has been pre-coated with an SLC13A2 antibody specific to SLC13A2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for SLC13A2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SLC13A2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SLC13A2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample collection and storage
Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20
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文献和实验[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
5-monooxygenase activation protein, eta polypeptide (YWHAH), mRNA 2195 NM_002939 Homo sapiens ribonuclease/angiogenin inhibitor (RNH), mRNA 726 NM_003562 Homo sapiens solute carrier family 25 (mitochondrial carrier; oxoglutarate carrier), member 11 (SLC
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