Rabbit ITIH3 ELISA KIT

Rabbit Inter- alpha- trypsin inhibitor heavy chain H3, ITIH3 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Inter-alpha-trypsin inhibitor heavy chain H3,ITI heavy chain H3,ITI-HC3,Inter-alpha-inhibitor heavy chain 3, ITIH3,H3P; ITI heavy chain H3; ITI-HC3; SHAP; inter-alpha (globulin) inhibitor H3; inter-alpha (globulin) inhibitor, H3 polypeptide; inter-alpha-inhibitor heavy chain 3; inter-alpha-trypsin inhibitor heavy chain H3; pre-alpha (globulin) inhibitor, H3 polypeptide; serum-derived hyaluronan-associated protein; inter-alpha-trypsin inhibitor heavy chain 3

Search name

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Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rabbit inter-alpha-trypsin inhibitor heavy chain h3,Inter-alpha-inhibitor heavy chain 3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inter-alpha-inhibitor heavy chain 3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Inter-alpha-inhibitor heavy chain 3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Inter-alpha-inhibitor heavy chain 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Inter-alpha-inhibitor heavy chain 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.