Rabbit Leptin ELISA Kit

Rabbit Leptin, LEP ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Leptin,Obese protein,Obesity factor , leptin,LEPD; OBS; leptin (murine obesity homolog); leptin (obesity homolog, mouse); obese protein;
obese, mouse, homolog of; obesity factor

Search name
Rabbit Leptin ELISA Kit, Rabbit LEP ELISA Kit, Rabbit Obese protein ELISA Kit, Rabbit Obesity factor ELISA Kit, Rabbit
LEPD ELISA Kit, Rabbit OBS ELISA Kit, Rabbit leptin murine obesity homolog ELISA Kit, Rabbit leptin obesity homolog
ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rabbit leptin,Leptin concentrations in serum, plasma, tissue
homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Leptin, During the reaction, Leptin in the sample or standard competes with a fixed amount of
biotin-labeled Leptin for sites on a pre-coated Monoclonal antibody specific to Leptin. Excess conjugate and unbound sample or standard
are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid
solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Leptin in the
samples is then determined by comparing the O.D. of the samples to the standard curve.