- ￥3480
- LSMBIO
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- CELI-66112Rb
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测兔血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
兔
- 标记物：
Rabbit Inositol trisphosphate,InsP3
- 样本：
兔血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rabbit Inositol trisphosphate, InsP3 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Inositol trisphosphate,Inositol 1,4,5-trisphosphate, triphosphoinositol,InsP3,Ins3P,IP3
Search name
Rabbit Inositol trisphosphate ELISA KIT ,Rabbit Inositol 1, 4, 5-trisphosphate ELISA KIT ,Rabbit triphosphoinositol ELISA KIT ,Rabbit InsP3 ELISA KIT ,Rabbit Ins3P ELISA KIT ,Rabbit IP3 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rabbit Inositol trisphosphate concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inositol trisphosphate, During the reaction, Inositol trisphosphate in the sample or standard competes with a fixed amount of biotin-labeled Inositol trisphosphate for sites on a pre-coated Monoclonal antibody specific to Inositol trisphosphate. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Inositol trisphosphate in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Rabbit-Inositol-trisphosphate-InsP3-ELISA-Kit
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文献和实验Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assay
Inositol(1,4,5)trisphosphate [Ins(1,4,5)P3 ] is an important second messenger that activates its cognate Ins(1,4,5)P3 receptor to release Ca2+ from intracellular stores. The assay described in this chapter uses the Ins(1,4,5)P3 receptor
a protocol for extracting and neutralizing plant samples and performing the receptor binding assay (using a commercially available kit). The protocol described has been used effectively to monitor InsP3 levels in plant tissues of different origin
Phosphoinositides, Inositol Phosphates, and Phospholipase C in Embryonic Stem Cells
, including cell growth and differen-tiation. Activation of hormone-sensitive PLC results in the rapid breakdown of polyphosphoinositides to generate two second messengers: inositol trisphosphate and dia-cylglycerol. The water-soluble inositol trisphosphate
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