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文献和实验Lentivirus-Based Virus-Like Particles as a New Protein Delivery Tool
Virus Like Particles (VLPs) are self-assembling, nonreplicating, nonpathogenic, genomeless particles similar in size and conformation to intact infectious virions. The possibility of engineering VLPs to incorporate heterologous polypeptides
Production and Purification of Hybrid Ty-VLPs
The self-assembly properties of a protein encoded by the TYA gene of the yeast Ty element can be exploited to produce hybrid Ty-VLPs (virus-like particles) (1 ,2 ). There has been developed a series of expression vectors that allow
Expression Vectors for the Construction of Hybrid Ty-VLPs
Ty, which self-assembles into virus-like particles (VLPs) (1 ,2 ). Additional protein coding sequences can be fused to the carrier protein gene and expressed in yeast to produce hybrid Ty-VLPs (3 ,4 ). The physical characteristics of the VLPs
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