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- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
无锡莱弗思生物实验器材有限公司
- 规格:
5 μg
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. The secreted recombinant GST-F2 /F1 protein was further analysed using glycosidases. Our results showed that the GST-F2 /F1 protein were sensitive to peptide: N -glycosidase F (PNGase F) treatment, but not to Endoglycosidase H (EndoH) treatment. This indicates
Deglycosylation of Glycoproteins Using Endoglycosidases
enzyme can be added. Note: For human transferrin, PNGase F and Endo F2 release all oligosaccharides in 1 hr. at 37 °C, whereas Endo F3 requires 16 hr. for 50% release of the one oligosaccharide chain. When large scale release of intact
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