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- 库存:
100
- 供应商:
无锡莱弗思生物实验器材有限公司
- 规格:
10 μg
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文献和实验Culturing Human Embryonic Stem Cells in Feeder-Free Conditions
% confluent (see Fig. 1 ). REFERENCES McElroy S.L., Reijo Pera R.A. 2008a. Preparation of mouse fibroblast feeder cells for human embryonic stem cell culture. CSH Protocols (this issue). doi: 10.1101/pdb.prot5041.[Abstract/Free
Protein Syntheses in Cell Free Systems
Materials •Suspension culture of fibroblast cells (1 liter)•35 mM Tris-HCl,pH 7.4,140 mM NaCl (TBS buffer)•10 mM Tris-HCl,pH 7.5,10 mM KCl,and 1.5 mM magnesium acetate (TBS-M)•10X TBS-M200 mM Tris-HCl,pH 7.5,1200 mM KCl,50 mM magnesium acetateand 70
Free-Flow Electrophoresis of the Human Urinary Proteome
Prefractionation of complex protein samples prior to mass spectrometry provides a method for the isolation of low-abundance proteins into specific fractions thereby enabling their identification. Free-flow electrophoresis in the isoelectric
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