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- 库存:
100
- 供应商:
无锡莱弗思生物实验器材有限公司
- 规格:
10 μg
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文献和实验Protein Syntheses in Cell Free Systems
equilibrated with 1X TBS-M buffer•Liquid nitrogen storage•Reaction mixture for protein synthesis,containing the following in a total volume of 50 µl 2Tris-HCl,pH 7.5 1.5 µmMg acetate 0.15 - 0.20 µmKCL 4.0 - 5.0 µm-Mercaptoethanol0.25 µmATP 0.05 µmGTP 0.005 µ
Carboxypeptidase Y (EC 3.4.16.1)
(no suitable chemical method exists for the sequential removal of C-terminal amino acids from a polypeptide). The protein or peptide being analyzed is digested with carboxypeptidase and aliquots removed at timed intervals, and analyzed for the presence of free
Culturing Human Embryonic Stem Cells in Feeder-Free Conditions
% confluent (see Fig. 1 ). REFERENCES McElroy S.L., Reijo Pera R.A. 2008a. Preparation of mouse fibroblast feeder cells for human embryonic stem cell culture. CSH Protocols (this issue). doi: 10.1101/pdb.prot5041.[Abstract/Free
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