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- 库存：
100
- 供应商：
无锡莱弗思生物实验器材有限公司
- 规格：
10 μg
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
type of interest, grown under appropriate cell culture conditions with appropriate medium Cells, bacterial, competent, high-efficiency ( 5 x 109 cfu/µg DNA) XL10-Gold ultracompetent cells (Stratagene) have produced optimal results.
1 ul DNA x ul [[gamma]]-[32-P]-rATP 10 uCi T4 polynucleotide kinase 1 ul (3U/ul) 10 ul [[gamma]]-[32-P]-rATP (35020) ICN and T4 polynucleotide kinase (70031) from United States Biochemicals. 2. Incubate at 37degC
Identification of a Mutant Kinase/ATP Analog Pair
, it is necessary to screen ATP analogs for their compatibility with the kinase mutant. This step requires an active form of the wild-type and mutant kinase, either as recombinant activated protein, or as protein that has been immunoprecipitated from transfected
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