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- 库存：
100
- 供应商：
无锡莱弗思生物实验器材有限公司
- 规格：
10 μL
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文献和实验Studies of the Ubiquitin Proteasome System
) 6% to 8% SDS‐PAGE minigels (unit 6.1 ) Anti‐ubiquitin antibody ( protocol 31 ; for immunoblot detection) Boiling water bath Additional reagents and equipment for SDS
Analysis of Protein Complexes by 2D Blue Native/SDSPAGE and Antibody-Shift Assay
Blue native polyacrylamide gel electrophoresis (BN-PAGE) is a powerful strategy for the separation of native multiprotein complex (MPC). Combined with other techniques, such as SDS–PAGE, immunoblotting, mass spectrometry, and antibody-shift
has eluted into the column. c) Add 5mL of PBS on top. d)Open Stopcock. collect in 2mL fractions, discarding the first 2mL and keeping the 2nd 2mL fraction. The second 2mL fraction contains the conjugated antibody*. *NOTE: Step d) appropriate only if 1mL
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