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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
无锡莱弗思生物实验器材有限公司
- 规格：
10 mg
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文献和实验Standard hybridization technique
The standard solution typically used for both pre-hybridisation and hybridisation is based
Stewart Laboratory Standard Operating Procedure
+ 0.5% IFS (make fresh!). Incubate at RT for 20 minutes. Wash cells once with 1 mL wash buffer. Pellet and resuspend in 0.1 M sodium tetraborate (Na2B4O7). Incubate at RT for 2 minutes. Wash cells twice with 1 mL wash buffer (if a negative control of PI
Standard Protocols Autoradiography (35S)
). The developer should be a 1:1 dilution in water. The fixer is 30% (w/v) of sodium thisulphate. Place slides in a dark box and carry to dark room. Also take a slide rack, tweezers and the emulsion. Switch on the red light and switch
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