Thymidine 5′-diphosphate sodiu

3′‐Modified Oligonucleotides and their Conjugates

GO TO THE FULL PROTOCOL: PDF or HTML at Wiley Online Library Table of Contents Basic Protocol 1: Preparation of 3′

Use of a Novel 5′‐Regioselective Phosphitylating Reagent for One‐Pot Synthesis of Nucleoside 5′‐Triphosphates from Unprotected Nucleosides

thymidine 5′‐triphosphate (TTP, 8t O ). The crude sample can be analyzed on a Welchrom C18 (or Ultisil C18) reversed‐phase column (4.6 × 250 mm) measured at 260 nm at a flow of 1.0 mL/min using a linear gradient of 0% to 40% buffer B in 20 min. Buffer A: 20

Biotinylation of a Propargylated Cyclic (3′‐5′) Diguanylic Acid and of Its Mono‐6‐Thioated Analog Under “Click” Conditions

Azidoacetic anhydride: bromoacetic acid (Aldrich) and sodium azide (Aldrich); Dyke et al. ( ) N,N ′‐Dicyclohexylcarbodiimide (Aldrich