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文献和实验Plasmid-Based Reporter Genes: Assays for Green Fluorescent Protein
spectral properties to its chromophore (2 ) that consists of a Ser65, Tyr66, and Gly67 tripeptide (3 ). Autocatalytic cyclization of this tripeptide, induced by oxidation of Tyr66, is a necessary posttranslational step for proper fluorescence (strong
and then cleaved with the highly selective TEV protease to remove the affinity tag and uncover an N-terminal cysteine. The N-terminal cysteine is then used in native chemical ligation reaction to join the IL-2 protein fragment to a glycosylated tripeptide thioester
Monitoring Glutathione Homeostasis in Nanoparticle-Treated Hepatocytes
This chapter describes a method for the analysis of human hepatocarcinoma cells (Hep G2 cells) for reduced and oxidized glutathione, following treatment with nanoparticle formulations. Glutathione is a tripeptide (l -γ-glutamyl-l -cysteinyl
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