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- 库存:
100
- 供应商:
无锡莱弗思生物实验器材有限公司
- 规格:
5 mg
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文献和实验, or obstructive jaundice: Hemolytic jaundice: When red blood cell lysis exceeds the liver's capacity to conjugate bilirubin (binding bilirubin to a polar group makes it water soluble and able to be excreted by the kidneys), hemolytic
the two reagents in pH 9.5 buffer just before use at the following ratio: 10 ml of pH 9.5 buffer 100 ul nBT 100 ul BCIP 0.5 M EDTA (disodium salt) 16.8 g/100 ml antimouse IgG (whole molecule) alkaline phosphatase conjugate (Sigma A5153
. Wash three times with PBS/Tween as before. 6.Add to each well 100 µl of substrate solution appropriate for the conjugate antibody used. For alkaline phosphatase conjugate, use p-nitrophenyl phosphate disodium (Sigma 104) dissolved in substrate buffer
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