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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
无锡莱弗思生物实验器材有限公司
- 规格：
1 mg
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文献和实验E.Z.N.A. Cycle-Pure Kit Spin Protocol
of the sample at 260nm and then at 280nm. The DNA concentration is calculated as follows: 260 DNA concentration = A x 50 x (Dilution Factor) ug/ml Fragments greater than 500bp in length can routinely be purified at > 80% yield. 260 Bands ranging
E.Z.N.A.® Yeast Plasmid Mini Spin Protocol
good yields. Incubate at 30°C for at least 30 min. 4. Pellet spheroblasts by centrifuging at 4,000 x g for 5 min at room temperature. Discard the supernatant completely. 5. Resuspend the spheroblasts pellet with 250 ul Buffer YP I/RNase
Protocol for Trichloroacetic Acid (TCA) Precipitation
and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish
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