相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
无锡莱弗思生物实验器材有限公司
- 规格:
50 mg
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Purification of Plasmid from 50 ml-culture
of cold 70% ethanol. Discard the fluid. 14. Add to the pellet 400 ul of TE containing DNase-free RNase A (20 ug/ml). Incubate the tube for 30 min at 37 C. 15. After 30 min, carefully check the content of the tube. If nucleic acid pellet
(10mg/ml); incubate at 37 ℃ for 10'. • Add 50 ml phenol/chloroform and spin for 2', remove 50 ml supernatant and add 10 ml 5 M NaCl plus 21 ml 40% PEG 8000. • Incubate on ice 5', spin 5' at room temperature and resuspend in 90 ml Q (for pUC ori
' at room temperature. • Wash with 80% EtOH, dry and resuspend in 60 ml TE plus 1 ml RNaseA (10mg/ml); incubate at 37 ℃ for 10'. • Add 50 ml phenol/chloroform and spin for 2', remove 50 ml supernatant and add 10 ml 5 M NaCl plus 21 ml 40% PEG
技术资料暂无技术资料 索取技术资料









