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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
无锡莱弗思生物实验器材有限公司
- 规格：
25 mg
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文献和实验Purification of the Constitutive Nitric Oxide Synthase
ligand is a structural mimic of the cofactor nicotinamide adenine dinucleotide phosphate (NADPH) that is required for NOS activity. Therefore, the biospecific elution is accomplished by the presence of NADPH. The purification of both constitutive isoforms
The MTT calorimetric assay determines the ability of viable cells to convert a soluble tetrazolium salt [3–(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) into an insoluble formazan precipitate. Tetrazolium salts accept
Growing feeder-independent embryonic stem cells§
before use. Trypsin solution: Add 100 mg of EDTA tetrasodium salt (Sigma) to 500 ml of PBS. Filter sterlize and add 10 ml (for 1×) or 20 ml (for 2×) of 2.5% trypsin solution (Gibco-BRL) and 5 ml of chicken serum (Gibco-BRL). Store in 20 ml aliquots
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