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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
IgG purified from mouse serum
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
-20°C
- 克隆性:
多克隆
- 标记物:
AF555-conjugated
- 适应物种:
Mouse
- 保质期:
12个月
- 抗原来源:
Mouse
- 目录编号:
CSB-PA288765
- 级别:
二抗
- 库存:
充足
- 供应商:
武汉益普生物科技有限公司
- 宿主:
Goat
- 应用范围:
ELISA, IH, IF, FC; Recommended dilution: ELISA:1:5000-1:10000, IH:1:100-1:1000, IF:1:100-1:1000, FC:1:100-1:4000
- 抗体英文名:
Goat Anti-Mouse IgG (H&L); AF555 conjugated
- 规格:
500μl/100μl
| 规格: | 500μl | 产品价格: | ¥1500.0 |
|---|---|---|---|
| 规格: | 100μl | 产品价格: | ¥500.0 |
| 产品名称 | Goat Anti-Mouse IgG (H&L); AF555 conjugated |
| 货号 | CSB-PA288765 |
| 抗体类型 | Secondary Antibody |
| 克隆类型 | Polyclonal |
| 免疫原种属 | Mouse |
| 免疫原 | IgG purified from mouse serum |
| 宿主 | Goat |
| 反应种属 | Mouse |
| 应用范围 | ELISA, IH, IF, FC Recommended dilution: ELISA:1:5000-1:10000, IH:1:100-1:1000, IF:1:100-1:1000, FC:1:100-1:4000 |
| 抗体亚型 | IgG |
| 标记方式 | AF555-conjugated |
| 产品提供形式 | Liquid |
| 纯化方式 | The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
| 保存缓冲液 | Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide |
| 储存条件 | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |

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文献和实验Anti-GFP/BrdU In Situ Immunostaining
IgG (Jackson ImmunoResearch, 111-096-006) Biotinylated anti-BrdU (AbCam, ab2284-125) Streptavidin-Alexa Flour 555 (Invitrogen/Molecular Probes, S21381) Goat serum Horse serum BSA 2N HCl DAPI Fluorescent mounting medium (Vectashield
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
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