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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
Immunotag™ Rat IMA (Ischemia Modified Albumin) ELISA
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
96wellplate
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文献和实验at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
saline (PBS) (Cat. no. 10010-049) or equivalent Optional: ArC™ Amine Reactive Compensation Bead Kit (Cat. no. A10346) 实验步骤 1. Using the AbC™ anti- Rat/Hamster Bead Kit 1) Completely resuspend the AbC™ RH
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
l/well of 1:625 Biotinylated Goat anti-rat IgG prepared by adding 32 µl IgG (Calbiochem #60653) to 20 ml blocking buffer with the addition of 200 µl of 10% Tween. Return plate to the 4°C on the rotator for 6 hours.• APPLYING STREPTAVIDINAfter 6 hours
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