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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
Immunotag™ Rat COL2 (Collagen Type II) ELISA
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
96wellplate
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文献和实验at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
Immunofluorescent Staining of Mouse and Rat Leukocytes
16 (Mouse BD Fc Block™; Cat No. 553141/553142) or anti-rat CD32 (Rat BD Fc Block™; Cat. No. 550270/550271) is recommended. BD Fc Block™ can be added to cells (~0.25 µg per million cells, 3 - 5 min, 4°C) and need not be washed out prior to addition of primary
3 and disproportionate micromelia (Dmm ) to α1 type II collagen (Col2a1 ) locus on chromosome 15. We have identified the mutation in cho , which is a deletion of a cytosine residue in the coding region of Col11a1 mRNA (1 ). In Dmm , a deletion of three nucleotides
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