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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
Immunotag™ Rat BDNF (Brain Derived Neurotrophic Factor) ELISA
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
96wellplate
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
Visualization and Quantitation of Neurotrophin mRNAs
). The neurons that survive this phase are those that form functional connections with their targets, competing successfully for a limited supply of a target-derived trophic factor (Barde, 1989). The concept of neurotrophic interactions
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