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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
Immunotag™ Human Nuclear receptor corepressor 2 ELISA Kit
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
96wellplate
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文献和实验Characterization of Nuclear Receptor Ligands by Multiplexed Peptide Interactions
, although described for nuclear receptors, may also be applied to other types of molecular interactions. Keywords: Microsphere; flow cytometry; nuclear receptor; coactivator; corepressor; cofactor; agonist; antagonist; ligand; ligand binding
at 4°C. Transfer supernatant (nuclear fraction) to a clean microcentrifuge tube. 11. Aliquot and store at –80°C. The nuclear extracts are ready for assay. 12. Quantitate protein concentration using the Quant-iT™ Protein Quantitation Kit (Cat. no. Q
should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice.An example
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