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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
Immunotag™ Rat 2',3'-cyclic-nucleotide 3'-phosphodiesterase ELISA Kit
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
96wellplate
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文献和实验Sample Protopcol for Two-Step Carbodiimide Coupling of Protein to MagPlex™ Magnetic Carboxylated Microspheres Microspheres should be protected from prolonged exposure to light throughout this procedure. 1.Resuspend
Procedure Dilute p27 monoclonal antibody 1:1000 (v/v) in Carbonate Coating Buffer. Add 100 µl/well and incubate o/n @ 4°C or 1 hr @37°C. Wash 3X with TBST (pour onto plate, empty into sink, hit onto towel 3x to clear wells
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
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