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- EVV33602
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- 保质期：
1 year
- 英文名：
Recombinant PIV5 HN/Hemagglutinin-neuraminidase Protein, C-His
- 库存：
999
- 供应商：
AntibodySystem
- 规格：
100 μg
| 稳定性和储存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt. |
| 种属 | Parainfluenza virus 5 (strain W3) (PIV5) (Simian virus 5) |
| 保存溶液 | Hemagglutinin-neuraminidase, 3.2.1.18, HN |
| 状态 | Lyophilized |
| 保存溶液 | Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒素水平 | Please contact with the lab for this information. |
| 表达系统 | Mammalian cells |
| Accession号 | P04850 |
| 蛋白长度 | Ser114-Ser565 |
| 性质 | Recombinant |
| 预测分子量 | 52.90 kDa |
| 重悬 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
AntibodySystem's product range supports research in multiple fields, encompassing 48 types of viruses, superbugs, parasites, oncology, Alzheimer's,Parkinson's,hypersensitivity,immune suppression,and immune activation.
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文献和实验Expression of Recombinant Cytochromes c in E. coli
for proper folding and stability. However, significant advances in expression of recombinant cytochromes c have been made during the last decade. It has been shown that a single gene cluster, ccmA–H , is responsible for cytochrome c maturation in Escherichia
. This purification process resulted in greater than 95% pure rBoNTC(Hc )-C based on SDS-PAGE, and yielded up to 0.2 g of rBoNTC(Hc )-C/kg cells. The final protein yield is a function of protein expression level during fermentation and the purification methods
【原创】Protein A Sepharose 之进化篇。。。
洗脱条件比较剧烈，最后收集的蛋白质很有可能变性，或者是复性困难。 这种洗脱条件剧烈的Protein A 柱结合的重组蛋白质A一般具有5个串联结构域：E、D、A、B、C。这种重组蛋白A虽然除去了不重要的非结合域，仅由5个结构域组成，每个域均可以和IgG的Fc段结合，但不同的域结合强度略有差异。因此洗脱条件不均一，而且经常需要较低的pH值。如果减少串联结构域的个数，并且采取同型结构域串联，就可以避免不同结构域与抗体Fc 段亲和性的差异，洗脱条件会温和而均一。为此，我们可以做一个试验，比较一下
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