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文献和实验Inverse PCR & Cycle Sequencing
both ends of PCR products recovered from PlacW and PZ strains: Splac2 and Sp1 - for use with the Plac4/Plac1 5' PCR primer combination with either PZ or PlacW P-elements; allows sequencing of both ends of the PCR fragment Spep
Denaturing Gradient Gel Electrophoresis (DGGE)
:: PCR amplification of wild-type and test DNA. Bacteriophage promoters (T7 and SP6) are incorporated into the 5' ends of the PCR primers and therefore into the PCR
Lipid analysis-Week 3: GAS LIQUID CHROMATOGRAPHY
Lipid analysis During this week, you will analyze the fatty acid composition of the individual lipid fractions recovered from the TLC plate. Gas chromatography is a very sensitive method for the separation and quantification
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