Rab3a antibody - 107 108 K.O.

Monoclonal Antibody Production Protocol

the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series

Production of Human Fab Antibody Fragments from Phage Display Libraries

with the desired binding properties (1 ) (see review in ref. 2 ). Initially, the system was used for mapping antibody epitopes by expression of large libraries (107 –108 ) of random peptides (3 –5 ). However, the approach has proved readily applicable

溶血空斑实验技术（Hemolytic antibody plaques formation test technique）

细胞悬液的制备（1）小鼠免疫 每只小鼠经尾静脉或腹腔注入上述SRBC悬液0.2ml；（2）脾细胞悬液的制备 将免疫后第四天的小鼠拉颈处死，解剖取出脾脏，放入含含Ca2+、Mg2+冷的pH7.2的PBS中漂洗后，去掉结缔组织，加入适量的PBS，用弯头镊子挤压脾细胞，稍静置，吸上清液至离心管中，1500r/min离心5min，弃上清后，定量加入PBS，混匀，按白细胞计数法计算脾细胞数，最后用PBS调整细胞数至1.00×107个/ml，一般每只鼠脾脏细胞数为1×108～1.5×108。2、倾注底层琼脂