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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
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- 英文名：
Immunotag™ Human Ribonucleoside-diphosphate reductase subunit M2 ELISA Kit
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
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- 规格：
96wellplate
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文献和实验Redox Property of Ribonucleotide ReductaseSmall Subunit M2 and p53R2
Human ribonucleotide reductase (RR) small subunits, M2 and P53R2, play key roles in forming RR holoenzyme and supplying nucleotide precursors for DNA replication and repair. Currently, we are studying the redox property, structure
and then centrifuge at 14,000 RPM for 3 minutes. Save 20µl of the elutes as (Flag-Input). Next, take the remaining ~80µl of elute to clean tubes containing 40µl of EZVIEW™ Red anti-Flag M2 affinity gel beads (Sigma™) which have been pre-treated with a wash in 250µl
should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice.An example
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