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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
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- 保质期:
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- 英文名:
Immunotag™ Bovine Metalloproteinase inhibitor 1 ELISA Kit
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
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- 规格:
96wellplate
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文献和实验-Buffer® or LowCross-Buffer® STRONG as sample diluent, PlateBlock™ shows very good performance in serological ELISA tests, such as assays for neutralizing SARS-CoV-2 antibodies (s. also Fig. 5). The many benefits of animal-free solutions Animal
with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
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