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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
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- 英文名:
Immunotag™ Human Cross-linked C-terminal telopeptide of Collagen alpha-1 (I) chain ELISA Kit
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
96wellplate
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文献和实验beads are washed (carefully as to not agitate the beads) 3 times with 2x wash buffer;After the last wash (2x wash buffer) the complexes are eluted in 100µl of 2x elute buffer at 65°C for 5 minutes (Figure 1b); The elutes are then reverse cross-linked
for 3 hours at 4°C with 500nM 5'biotin end-labeled siRNA under gentle agitation, i.e. a rocking plate (Figure 1a). We utilized the EF1 alpha specific siRNA, EF52, shown previously to direct TGS in human cells (Morris et al. 2004). The EF52 siRNA
dividing cells and in several human tumors [18]. Fusion of the C-terminal polo-box from Plk-1 to penetratin (Table 2) led to a significant decrease in cell proliferation in different human cancer cell lines [19]. When peptides are introduced to the cell
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