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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
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- 英文名:
Low Speed Centrifuge, 220V, Plug Type E-Europe
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
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- 规格:
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文献和实验Transformation of E. coli by Electroporation
Materials: SOB medium E. coli host strain such as DH5α WB tRNA 5 M ammonium acetate
in a low speed centrifuge at 2000 rpm for 5 minutes. If I ligate 100 ng of blunted dephosphorylated vector to 50 ng of a blunt 500 bp PCR in a 30 µl reaction, I'll usually transform 5 ul into cells and plate out the whole tranformation, I normally
1.Add colony of desired bacterium to 25 mL LB flask; grow overnight. 2.Add 1 mL from overnight culture to 250 mL LB flask; grow until bacteria are in log phase (4-5 hours for E. coli) 3.Split culture across two centrifuge flasks
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