- ￥2816.10
- G-Biosciences
- 美国
- BT301-E
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
Lid Wet/Tank Blotting System for BT301
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
Each
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文献和实验Northern Blotting方法(Howell Lab)
.A. = [(cpm of washed) x (volume of labelling reaction)]/µg amount of DNA If S.A. is over 1x108, the probe is OK. Boil the probe for 5 min and chill it on ice for 5 min. Up/down spin the tube to bring down the condensation on the lid.
Western Blotting Using the SemiPhor Semi-Dry Transfer Unit
for orientation. Pre-wet the membrane in transfer buffer for 2 to 5 minutes.4.Place a mylar mask in the bottom of the SemiPhor unit. The mask should have a central, square opening cut in it that is 2 mm smaller than the gel in both length and height.5.In a dish
the membrane (Immobilon-P/PVDF) and 6 pieces of blotting paper (e.g. Schleicher and Schuell GB002) to the same size as the gel (~8.5 x 5.7 cm). Wet the membrane for 15 seconds in 100% methanol, then soak for 2’ in water, then equilibrate the membrane
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