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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
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- 保质期:
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- 英文名:
Incubator, Dry Bath (Mini) with cooling fan and 15 x 1.5ml Block, 220V, Plug Type I-Australia
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
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文献和实验by swirling, transfer to 1.5 ml microcentrifuge tubes, and incubate in an ice-water bath for 15 minutes. 5. Clear the lysate of precipitated SDS, proteins, membranes, and chromosomal DNA by centrifugation at 12,000 rpm for 15 minutes in a microcentrifuge
. 5. Dry the pellet for 5 minutes and then resuspend in MOPS-Acid buffer. 6. Anneal the primers by heating the solution for 2 minutes at 65℃ followed by slow cooling to room temperature for 15-30 minutes. 7. Perform sequencing reactions, using modified T
by either the mini- or midi-prep diatomaceous earth method, is mixed with primer, placed in a boiling-water bath, and rapidly cooled by plunging into an ethanol/dry-ice bath (28). Following an incubation on ice, the remaining sequencing extension reagents (reaction
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