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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
AP labeled goat α-rat IgG (0.6mg/ml)
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
1ml
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文献和实验Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
and can lower the titer. Autoclave the NZY top agar and place in a 50°C water bath for later use. Serial dilute the rat d9 decidual cDNA library (from Stratagene with a titer of 1010 pfu/ml) with SM buffer to 5 x 105 , 5 x 103 , 5 x 102 , 5 x 101
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
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