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- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
Calmodulin Elution Buffer (50mM Tris.HCl (pH7.5), 200mM NaCl, 2mM EGTA)
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
100ml
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文献和实验How to make DEPC-treated water and Tris Buffer
first, and then dissolve Tris to make the appropriate buffer. Trace amounts of DEPC will modify purine residues in RNA by carboxymethylation. Carboxymethylated RNA is translated with very low efficiency in cell-free systems. However, its ability to form DNA:RNA or RNA:RNA
Purification of Calmodulin from Bovine Brain
temperature. 3.2 M Tris Base stored at 4℃. The following buffers are made up the day before and kept at 4℃: 4.Homogenizing buffer- 4 liters of 50 mM MOPS,pH 7.0,1 mM 2-mercaptoethanol,1mM EDTA. 5.Dialysis buffer- 6.8 liters of 10 mM Tris,pH 7.5,50 mM NaCl
I: 0.5ml A + 0.5ml C + 0.83ml D + 0.1ml BSA + 35µl bME + 3.04ml H2O1X concentration:Enzyme: [Tris-HCl]. [MgCl2], [NaCl]Ban I: 6mM pH 7.4, 6mM, 6mM, 6mM bMEBg l II: 10mM pH 7.4, 10mM, 100mM, 10mM bMEBst N I: 10mM pH 8.0, 10mM, 150mMDra I: 10mM pH 8.0, 10mM
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